Hi,
Yes, only one of these option "should" be used, it never make sense to do the same thing on Update and on Late Update for example.
the "per seconds" option in the various actions is to provide an animation system that is guaranteed to work the same regardless of the performances ( FPS fluctuations), that's all there is to it. So instead of saying:
"move the gameobject by 10 units every frame"
you actually say
"move the gameobject by 10 units every seconds"
which implies that it will be consistent and predictable.
bye,
Jean